DNA体内转染EntransterTM-in vivo

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产品介绍

EntransterTM-in vivo是英格恩生物公司(Engreen Biosystem Co,Ltd.)最新研发合成的用于动物体内转染的转染试剂。EntransterTM-in vivo动物体内转染试剂可将DNA转染到动物体内,在动物体内进行蛋白表达实验。

EntransterTM-in vivo试剂不是病毒,改变了病毒转染方法复杂、周期长、不稳定、花费大、安全性等诸多问题。EntransterTM-in vivo转染效率高,方法简单,无需病毒包装,直接将DNA送入目的细胞的细胞核中表达。

EntransterTM-in vivo,非脂质体,采用纳米技术合成,通过保护和浓缩核酸,避免核酸被体液破坏,并通过物理作用将核酸富集在细胞表面,增强核酸进入细胞组织,从而产生作用。EntransterTM-in vivo能耐受体内复杂的环境,不产生免疫反应。

推荐应用范围

专用于动物体内转染,可直接转染DNA到动物体内进行:

● 基因治疗的动物体内研究

● 蛋白功能的动物体内研究

主要特点

最快3天可以得到结果,核酸+转染试剂,注射动物,完成转染。

● 肝、肾、心、肺,血液系统,神经系统,生殖系统,皮肤…成功应用。

● 可方便、快捷更换不同的DNA进行动物试验。

● 方法先进,抛弃病毒,众多实验室应用,稳定可靠。

● 比使用病毒节省90%以上费用,时间缩短到3天!

方法简单

如图1所示,只需要将核酸和转染试剂分别稀释,混合,无需过滤,即可直接注射动物。

 

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应用本产品文献

发表的部分文章分器官导读

在线查看文章:https://files.engreen.info/share/detail/f60c7b80-8d95-46d8-5f6b-1bb9212c1197 提取码:v6at

序号 研究方向 实验材料 文章题目 期刊
大脑
1 脑损伤 DNA Rbfox-1 contributes to CaMKIIa expression and intracerebral hemorrhage-induced secondary brain injury via blocking micro-RNA-124 Journal of Cerebral Blood Flow &Metabolism
2 脑损伤 DNA Critical role for Annexin A7 in secondary brain injury mediated

by its phosphorylation after experimental intracerebral hemorrhage in rats

Neurobiology of Disease
3 大脑海马 DNA Activating Transcription Factor 6 reduces Aβ1–42 and restores memory in Alzheimer’s disease model mice International Journal of Neuroscience
4 脑损伤 DNA CX3CL1/CX3CR1 axis attenuates early brain injury via promoting the delivery of exosomal microRNA-124 from neuron to microglia after subarachnoid hemorrhage Chen et al. Journal of Neuroinflammation
5 脑损伤 DNA Sodium/Hydrogen Exchanger 1 Participates in Early Brain Injury after Subarachnoid

Hemorrhage both in vivo and in vitro via Promoting Neuronal Apoptosis

Cell Transplantatio
6 脑损伤 DNA Exploration of MST1-Mediated Secondary Brain Injury Induced by Intracerebral Hemorrhage in Rats via Hippo Signaling Pathway Translational Stroke Research
7 脑损伤 DNA RAB7L1 Participates in Secondary Brain Injury Induced by Experimental Intracerebral Hemorrhage in Rats Journal of Molecular Neuroscience
8 脑损伤 DNA Transient receptor potential channel 1/4 reduces subarachnoid hemorrhage-induced early brain injury in rats via calcineurinmediated NMDAR and NFAT dephosphorylation Scientific Reports
9 脑损伤 DNA Gasdermin D serves as a key executioner of pyroptosis in experimental cerebral ischemia and reperfusion model both in vivo and in vitro J Neurosci Res
10 大脑神经 DNA NL1 expression level in Nrx1β and the excitability of PV interneurons in mice with POCD EXPERIMENTAL AND THERAPEUTIC MEDICINE
11 大脑神经 DNA DNA -“TMEM175 mediates Lysosomal function and participates in neuronal injury induced

by cerebral ischemia-reperfusion

Molecular Brain
12 大脑神经 DNA Galectin‑9 Promotes Neuronal Restoration via Binding TLR‑4 in a Rat Intracerebral Hemorrhage Model NeuroMolecular Medicine
13 大脑神经 DNA Cerebral cavernous malformation 3 relieves subarachnoid hemorrhage-induced neuroinflammation in rats through inhibiting NF-kB signaling pathway Brain Research Bulletin
支气管
1 哮喘 DNA Bergenin, a PPARγ agonist, inhibits Th17 differentiation and subsequent neutrophilic asthma by preventing GLS1-dependent glutaminolysis Acta Pharmacologica Sinica
1 非小细胞癌 DNA Targeting LunX inhibits non-small-cell lung cancer growth and metastasis Cancer research
2 急性呼吸窘迫综合征 DNA Contribution of Thrombospondin-1 and -2 to Lipopolysaccharide-

Induced Acute Respiratory Distress Syndrome

Mediators of Inflammation
肝脏
1 肝再生 DNA Tmub1 negatively regulates liver regeneration via inhibiting STAT3 phosphorylation Cellular Signalling
2 乙肝 DNA Hepatitis B Virus Can Be Inhibited by DNA Methyltransferase 3a

via Specific Zinc_Finger_Induced Methylation of the X Promoter

BIOCHEMISTRY
胰腺
1 胰岛素

(糖尿病)

DNA Angptl7 promotes insulin resistance and type 2 diabetes mellitus by multiple mechanisms including SOCS3-mediated IRS1 degradation The FASEB Journal
1 肠炎 DNA Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation Acta Pharmaceutica Sinica B
2 肠缺血 mtDNA mtDNA-STING pathway promotes necroptosis-dependent enterocyte injury in intestinal ischemia reperfusion Cell Death and Disease
1 骨质疏松 DNA Extracellular regulated kinase 5 mediates osteoporosis through modulating viability and

apoptosis of osteoblasts in ovariectomized rats

Bioscience Reports
2 骨关节炎 DNA Clock Mutant Promotes Osteoarthritis by Inhibiting the Acetylation of NFKB Osteoarthritis and Cartilage
肌肉
1 骨骼肌 DNA Circular RNA circMYBPC1 promotes skeletal muscle differentiation by targeting MyHC Molecular Therapy: Nucleic Acids
免疫
1 慢性荨麻疹 DNA SELE Downregulation Suppresses Mast Cell Accumulation to Protect against Inflammatory Response in Chronic Idiopathic Urticaria Int Arch Allergy Immunol
2 Th17细胞 DNA The role of GLS1-mediated glutaminolysis/2-HG/H3K4me3 and GSH/ROS signals in Th17 responses counteracted by PPARγ agonists Theranostics

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  1. EntransterTM系列转染试剂的成分和原理,化学结构,粒径,电位等数据

  2. siRNA转染与DNA转染有什么不一样?

  3. 体内转染常见问题

  4. 体内转染和病毒感染的比较

  5. 转基因动物/基因敲除动物与体内转染的比较

  6. 小鼠尾静脉注射技巧

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北京英格恩生物科技有限公司对本产品实行严格质量检验,并进行转染验证,以确保产品质量。请用户使用前务必认真阅读操作手册。如用户仍然有问题,请立即联系本公司人员,英格恩公司将负责提供全程技术服务。

使用限制

本品仅限科研用途。

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    DNA体内转染EntransterTM-in vivo
    货号:

    18668-11-2

    规格:

    0.5ml

    1 X ¥2,599.00 = ¥2,599.00