本文采用的英格恩产品: RNA-Entranster-invivo, 体内转染
P2Y12 shRNA treatment relieved HIV gp120-induced neuropathic pain in rats
Affiliations
- 1 Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People’s Republic of China.
- 2 Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People’s Republic of China; Nursing College, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China.
- 3 Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People’s Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China.
- 4 Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People’s Republic of China. Electronic address: liangsd@hotmail.com.
- PMID: 28827078
- DOI: 10.1016/j.neuint.2017.08.006
Abstract
Human immunodeficiency virus (HIV) envelope glycoprotein (glycoprotein 120, gp120) can induce chronic neuropathic pain by directly stimulating primary sensory afferent neurons. Activation of satellite glial cells (SGCs) in dorsal root ganglia (DRG) plays an important role in the transmission of neuropathic pain. The P2Y12 receptor is expressed in SGCs of DRG. In this study, we investigated the role of the P2Y12 receptor in HIV gp120-induced neuropathic pain. The results showed that peripheral nerve exposure to HIV gp120 increased mechanical and thermal hyperalgesia in gp120-treated model rats. The gp120 treatment increased the expression of P2Y12 mRNA and protein in DRG SGCs. Treatment with P2Y12 short hairpin RNA (shRNA) in DRG SGCs decreased the upregulated expression of P2Y12 mRNA and protein in DRG SGCs as well as relieved mechanical and thermal hyperalgesia in gp120-treated rats. Reduction of P2Y12 receptor decreased co-expression of P2Y12 and glial fibrillary acidic protein (GFAP), expression of GFAP, interleukin (IL)-1β, tumor necrosis factor (TNF)-receptor 1 (TNF-R1), and phosphorylation of Akt (p-Akt) proteins in DRG of gp120-treated rats. Upregulation of GFAP is a marker of SGC activation. Therefore, P2Y12 shRNA treatment decreased HIV gp120-induced mechanical and thermal hyperalgesia in gp120-treated rats.
Keywords: Dorsal root ganglia; HIV gp120-associated neuropathic pain; P2Y(12) receptor; Satellite glia cells.
