CCAA试剂盒，Cell Cycle and Apoptosis Analysis Kit，是基于PI染色(Propidium staining)方法进行细胞周期与细胞凋亡分析的试剂盒。
PI (Propidium，碘化丙啶) 是一种可对DNA染色的细胞核染色试剂，在嵌入双链DNA后释放红色荧光，其强度与DNA含量成正比。细胞内的DNA被PI染色后，可以用流式细胞仪对细胞进行DNA含量测定，根据DNA含量的分布情况，进行细胞周期和细胞凋亡分析。
本试剂盒通常用于贴壁或悬浮细胞的周期与凋亡检测。如果用于组织，则需要把组织消化成单细胞状态，再可以进行检测。
PI染液即开即用，无需再用缓冲液配制。
PI染液4℃存放，无需解冻，方便随时取用。
不需要提前用RNA酶消化，超快，省时。
| 问题 | 可能原因 | 解决方案 |
| G0/G1漂移或者突然变化 | 流式细胞仪通道有气泡或者被堵塞 | 检查流式细胞仪 |
| PI的浓度太低 | 提高PI浓度 | |
| 细胞与PI的孵育时间太短 | 增加孵育时间 | |
| 峰过宽 | 流式细胞仪流速太快 | 调整流式细胞仪 |
| 样品有气泡 | 去除气泡 | |
| 样品有死细胞 | 采用新样品 | |
| 无峰 | 坏死细胞过多 | 预先在显微镜下观察，并用新样品替代 |
| 样品中没有细胞核 | ||
| 细胞碎片太多 |
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<本品低温运输，储存于-20℃，有效期12个月 ；可4℃存放，有效期1个月。
PI (碘化丙啶)对人体有刺激性，请穿实验服并戴一次性手套操作。
北京英格恩生物科技有限公司本产品实行严格质量检验，并进行转染验证，以确保产品质量。请用户使用前务必认真阅读操作手册。如用户仍然有问题，请立即联系本公司人员，英格恩公司将负责提供全程技术服务。
本品仅限科研用途。
CCAA 凋亡检测试剂盒能检测什么?
基于 PI(碘化丙啶)染色,对细胞周期与细胞凋亡进行分析。
CCAA 试剂盒的检测原理是什么?
PI 嵌入双链 DNA 后释放红色荧光,强度与 DNA 含量成正比,通过测定 DNA 含量及其分布来判断细胞周期和凋亡情况。
CCAA 试剂盒用什么仪器检测?适用哪些样本?
用流式细胞仪测定 PI 染色后的 DNA 含量;通常用于贴壁或悬浮细胞,若为组织需先消化成单细胞状态再检测。
CCAA 试剂盒使用方便吗?
PI 染液即开即用、无需缓冲液配制,4℃ 存放无需解冻,且不必提前用 RNA 酶消化,超快省时。
CCAA 试剂盒如何运输储存?有哪些规格?
低温运输,-20℃ 储存有效期 12 个月,4℃ 存放有效期 1 个月;货号 EC020,规格 20ml。
