Journal of Functional Foods Volume 52, January 2019, Pages 587-595

Stevia residue extract ameliorates oxidative stress in d-galactose-induced aging mice via Akt/Nrf2/HO-1 pathway

Author links open overlay panelLeiZhaoabHuaqingYangabMeiliXucXinWangabChengtaoWangabYunheLiancArshadMehmoodabHaochenDaid

aBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, ChinabBeijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinacChenguang Biotech Group Co., Ltd., Handan 057250, Hebei, ChinadDepartment of Food Science, University of Massachusetts, Amherst, MA 01003, USA


The present study investigated the effect of Stevia residue extract (SRE) against oxidative stress in d-galactose (d-gal) induced aging mice. LC-MS/MS analysis revealed that SRE is a good source of chlorogenic acids. Biochemical results showed that SRE significantly increased the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and total antioxidant capacity (T-AOC), and decreased acetylcholinesterase (AChE) activity and malondialdehyde (MDA) level in serum, liver or brain of d-gal induced aging mice. At 200 mg/kg, SRE up-regulated the mRNA expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream target genes including GPx1, CAT, SOD1, quinone oxidoreductase-1 (NQO1) and heme oxygenase-1 (HO-1) in d-gal treated mice liver. SRE also up-regulated the protein expression of Nrf2, HO-1 and the ratio of phosphorylated Akt to Akt (pAkt/Akt) in d-gal treated mice liver. These findings suggest that SRE is able to protect against oxidative stress in d-gal induced aging model via activation of Akt/Nrf2/HO-1 pathway. In conclusion, SRE may provide a promising dietary approach for the prevention or alleviation of oxidative stress and age-related conditions.


SteviaChlorogenic acidsHydroxycinnamate derivativesd-galactoseAgingOxidative stress