Biochem Pharmacol.2020 Apr;174:113822.doi: 10.1016/j.bcp.2020.113822. Epub 2020 Jan 25.

MicroRNA let-7g-5p alleviates murine collagen-induced arthritis by inhibiting Th17 cell differentiation

Pei Yang  1 Mingfei Zhang  1 Xiang Wang  1 A-Lan Xu  1 Meiyu Shen  1 Baoping Jiang  1 Xueping Zhou  2 Lingling Zhou  3 Affiliations

Abstract

Rheumatoid arthritis (RA) is a chronic and systemic autoimmune disease with complicated pathogenesis. IL-17-producing T helper cells (Th17) are important players in the RA process. Despite numerous researches have proven that microRNAs (miRNAs) are crucial to regulate autoimmune diseases including RA, the effect of miRNAs on Th17 cell differentiation and function in the RA progress is not clear. Here, our results showed that the expression of miRNA let-7g-5p was substantially lower in RA patients and CIA mice compared with healthy controls, accompanied by the increased Th17 cell population. Furthermore, the inhibition of let-7g-5p on Th17 cell differentiation and function were verified in vitro. Notably, the disease severity in CIA mice was significantly alleviated after the treatment of let-7g-5p mimics. In addition, let-7g-5p mimics treatment markedly down-regulated the frequency of Th17 cells in CIA mice. Taken together, our findings indicate that let-7g-5p can ameliorate CIA through blocking the differentiation of Th17 cells, which may be a novel strategy to treat autoimmune diseases such as RA.

Keywords: Collagen‑induced arthritis; Let-7g-5p; Rheumatoid arthritis; Th17 cells.

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