Aging (Albany NY).2020 Jul 9;12(13):13234-13254.doi: 10.18632/aging.103431.Epub 2020 Jul 9.

本文采用的英格恩产品: RNA-Entranster-invivo

Increasing the expression of microRNA-126-5p in the temporal muscle can promote angiogenesis in the chronically ischemic brains of rats subjected to two-vessel occlusion plus encephalo-myo-synangiosis

Chuan Chen  1 Cong Ling  1 Jin Gong  1 Chao Li  2 Liying Zhang  2 Shuangqi Gao  1 Zhangyu Li  1 Tengchao Huang  1 Hui Wang  1 Ying Guo  1 Affiliations

Free PMC article

Abstract

Background: miR-126-5p plays an important role in promoting endothelial cell (EC) proliferation. We thus explored whether miR-126-5p can promote EC proliferation and angiogenesis in chronically ischemic brains (CIBs).

Results: Improved revascularization in moyamoya patients was correlated with upregulated miR-126-5p expression in the TM and DM. In vitro experiments showed that miR-126-5p promoted EC proliferation through the PI3K/Akt pathway. CIBs from the agomir group exhibited significantly higher p-Akt, VEGF, CD31 and eNOS expression compared with the control CIBs. The ICBP and the RCF were significantly better in the agomir compared with the control group.

Conclusion: Increasing miR-126-5p expression in the TM can promote EC proliferation and angiogenesis in CIBs of 2VO+EMS rats through the PI3K/Akt pathway.

Methods: We assessed the correlation between revascularization and miR-126-5p expression in the temporal muscle (TM) and dura mater (DM) of moyamoya patients. The effect of miR-126-5p on EC proliferation and downstream signaling pathways was explored in vitro. We established an animal model of two-vessel occlusion plus encephalo-myo-synangiosis (2VO+EMS), transfected the TM with miR-126-5p agomir/antagomir, compared the expression of miR-126-5p and relevant downstream cytokines in brain tissue among different groups, and investigated the improvement in cerebral blood perfusion (ICBP) and the recovery of cognitive function (RCF).

Keywords: angiogenesis; encephalo-myo-synangiosis; endothelial cell proliferation; microRNA-126-5p; moyamoya disease.

在线客服
在线客服
热线电话
 
0
    0
    我的购物车
    购物车是空的去下单