The Targeting Effect of Cetuximab Combined with PD-L1 Blockade against EGFR-Expressing Tumors in a Tailored CD16-CAR T-Cell Reporter System
Yijian Li 1 2 , Qianqian Gao 2 , Huan Liu 2 , Shufen Lin 2 , Huanyi Chen 2 , Renpeng Ding 2 , Ying Gu 2 , Cheng-Chi Chao 2 , Xuan Dong 2 3 Affiliations
- PMID: 33646061
- DOI: 10.1080/07357907.2021.1894570
The switchable chimeric antigen receptors (CARs) have shown many advantages in CAR T-cell therapy. However, human primary T-cells are required to evaluate antigen-specific adaptors by IFN-γ assay or FACS analysis, which limits the throughput of adaptor screening. A sensitive and robust CD16-CAR Jurkat NFAT-eGFP reporter system has been developed to assess the therapeutic efficacy of antibody-targeted CAR-T-cell by effectively evaluating the T-cell activation by various tumor cells and the impact of immune checkpoint inhibitor antibodies. This reporter system facilitates the screening of targeted antibodies in a high throughput manner for the development of improved T-cell immunotherapy.
Keywords: CD16-CAR; PD-L1; cetuximab; immunotherapy.