USP5 attenuates NLRP3 inflammasome activation by promoting autophagic degradation of NLRP3

Baoshan Cai  ¹ , Jian Zhao  ¹ , Yuling Zhang  ¹ , Yaxing Liu  ¹ , Chunhong Ma  ¹ , Fan Yi  ² , Yi Zheng  ¹ , Lei Zhang  ¹ , Tian Chen  ³ , Huiqing Liu  ² , Bingyu Liu  ¹ , Chengjiang Gao  ¹ Affiliations

• PMID: 34486483
• DOI: 10.1080/15548627.2021.1965426

Abstract

3-MA: 3-methyladenine; AIM2: absent in melanoma 2; ATG5: autophagy related 5; BafA1: bafilomycin A₁; CASP1: caspase 1; CHX: cycloheximide; Co-IP: co-immunoprecipitation; CQ: chloroquine; DUBs: deubiquitinases; IL1B/IL-1β: interleukin 1 beta; LAMP1: lysosomal associated membrane protein 1; LPS: lipopolysaccharide; MARCHF7/MARCH7: membrane associated RING-CH-type finger 7; NFKB/NF-κB: nuclear factor kappa B; Nig.: nigericin; NLRC4: NLR family CARD domain containing 4; NLRP3: NLR family pyrin domain containing 3; PECs: peritoneal exudate cells; PMN: polymorphonuclear; PMs: peritoneal macrophages; PYCARD/ASC: PYD and CARD domain containing; TLRs: toll like receptors; TNF/TNF-α: tumor necrosis factor; Ub: ubiquitin; USP5: ubiquitin specific peptidase 5; WT: wild type.

Keywords: Autophagy-lysosome pathway; MARCHF7; NLRP3 inflammasome; USP5; deubiquitinase.