J Cell Mol Med. 2021 Jul;25(13):6242-6257.doi: 10.1111/jcmm.16579. Epub 2021 Jun 15.

中文速读

本研究针对衰老相关骨质疏松，使用C57BL/6小鼠模型，通过股骨骨膜内注射或颅骨皮下注射MACF1过表达质粒（PEGFP-C1A-ACF7）与EntransterTM In Vivo转染试剂复合物，以及体外使用EntransterTM-H4000转染试剂进行荧光素酶报告基因实验，验证MACF1通过抑制HES1促进骨形成，改善骨质疏松。

小鼠皮下骨质粒DNA

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MACF1 alleviates aging-related osteoporosis via HES1

Chong Yin^{1 2 3 4}, Ye Tian¹, Lifang Hu¹, Yang Yu⁵, Zixiang Wu¹, Yan Zhang¹, Xue Wang¹, Zhiping Miao¹, Airong Qian¹

Affiliations

¹ Lab for Bone Metabolism, Xi’an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences and Biotechnology, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, China.
² Lab of Epigenetics and RNA Therapy, Department of Clinical Laboratory, Academician (Expert) Workstation, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
³ Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, China.
⁴ Translational Medicine Research Center, North Sichuan Medical College, Nanchong, China.
⁵ Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, China.

PMID: 34133068
PMCID: PMC8366449
DOI: 10.1111/jcmm.16579

Abstract

Ageing-related osteoporosis is becoming an emerging threat to human health along with the ageing of human population. The decreased rate of osteogenic differentiation and bone formation is the major cause of ageing-related osteoporosis. Microtubule actin cross-linking factor 1 (MACF1) is an important cytoskeletal factor that promotes osteogenic differentiation and bone formation. However, the relationship between MACF1 expression and ageing-related osteoporosis remains unclear. This study has investigated the expression pattern of MACF1 in bone tissues of ageing-related osteoporosis patients and ageing mice. The study has further elucidated the mechanism of MACF1 promoting bone formation by inhibiting HES1 expression and activity. Moreover, the therapeutic effect of MACF1 on ageing-related osteoporosis and post-menopausal osteoporosis was evaluated through in situ injection of the MACF1 overexpression plasmid. The study supplemented the molecular mechanisms between ageing and bone formation, and provided novel targets and potential therapeutic strategy for ageing-related osteoporosis.

Keywords: HES1; MACF1; ageing; bone formation; osteoporosis.

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