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RNA转染试剂EntransterTM-R

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产品介绍

EntransterTM-R是英格恩生物（Engreen Biosystem）研发合成的针对siRNA、microRNA、mimic、inhibitor、mRNA和shRNA等RNA的转染试剂。EntransterTM-R不仅可以转染小RNA，而且针对mRNA等长链RNA优化。由于纳米技术的应用，EntransterTM-R4000能做到高达90%的转染效率和60-90%的沉默效率，对难转染细胞和原代细胞也非常适用。

推荐应用范围

小RNA转染。包括siRNA转染，miRNA，mimic，inhibitor等。 长链的mRNA和shRNA转染，针对mRNA和长链的shRNA等RNA转染优化。 可转染动物细胞、昆虫细胞等，也可以转染原代细胞、悬浮细胞和原代细胞等传统难转染细胞。

主要特点

纳米聚合物，转染效果优于脂质体转染试剂 高效转染，沉默效率高，可以达到90％以上的沉默效率 低细胞毒性，有血清转染，无须反复更换培养基 原代细胞、悬浮细胞和敏感细胞都能转。

  1. 3D Biomimetic Calcified Cartilaginous Callus that Induc […]

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  4. Immunopharmacol Immunotoxicol. 2022 Jun 23;1-13.doi: 10.1080/08923973.2022.2078727. Online ahead of print. (IF2.73) LncRNA DLEU2 silencing impedes the migration, invasion and EMT in gastric cancer cell by suppressing PI3K/AKT signaling pathway LncRNA 沉默通过抑制PI3K/AKT信号通路阻止胃癌细胞的迁移、侵袭和EMT

  5. Bioengineered. 2022 May;13(5):11503-11516.doi: 10.1080/21655979.2022.2068737. (IF3.269) TNF-like weak inducer of apoptosis / nuclear factor κB axis feedback loop promotes spinal cord injury by inducing astrocyte activation 通过诱导星形胶质细胞活化促进脊髓损伤

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  8. Life Sci.2022 Mar 3;120458.doi: 10.1016/j.lfs.2022.120458.dentification of the upstream regulators of KDM5B in gastric cancer

  9. Arch Virol. 2022 Feb 24.doi: 10.1007/s00705-022-05397-8.Identification of endoplasmic-reticulum-associated proteins involved in Bombyx mori nucleopolyhedrovirus entry by RNA-seq analysis

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  16. Cancer Gene Ther. 2021 Jun;28(6):667-678.doi:10.1038/s41417-020-00247-8. Epub 2020 Oct 27. circ_001504 promotes the development of renal cell carcinoma by sponging microRNA-149 to increase NUCB2促进肾细胞癌的发展

  17. Exp Ther Med.2021 Nov;22(5):1321.doi: 10.3892/etm.2021.10756.Epub 2021 Sep 20.Gas6/Axl signaling pathway promotes proliferation, migration and invasion and inhibits apoptosis in A549 cells. Gas6/Axl信号通路促进A549细胞增殖、迁移和侵袭并抑制凋亡

  18. Exp Ther Med.2021 Nov;22(5):1311.doi: 10.3892/etm.2021.10746. Epub 2021 Sep 16.Blocking tumor necrosis factor-α delays progression of chronic obstructive pulmonary disease in rats through inhibiting MAPK signaling pathway and activating SOCS3/TRAF1

  19. Dig Dis Sci. 2021 Sep;66(9):3036-3044.doi:10.1007/s10620-020-06640-5. Epub 2020 Oct 22.Knockdown of Bcl-2-Associated Athanogene-3 Can Enhance the Efficacy of BGJ398 via Suppressing Migration and Inducing Apoptosis in Gastric Cancer 胃癌

  20. Dis Markers. 2021 Sep 3;2021:5594659.doi: 10.1155/2021/5594659. eCollection 2021.Differential Expression of Long Noncoding RNAs Reveals a Potential Biomarker for Intractable Pemphigus难治性天疱疮的潜在生物标志物

  21. J Invertebr Pathol. 2021Sep;184:107647.doi:10.1016/j.jip.2021.107647. Epub 2021 Jul 23.Bombyx mori Apolipophorin-III inhibits Beauveria bassiana directly and through regulating expression of genes relevant to immune signaling pathways家蚕载脂蛋白III通过调节免疫信号通路相关基因的表达直接抑制白僵菌

  22. Front Physiol. 2021 Aug 4;12:663482. doi: 10.3389/fphys.2021.663482. eCollection 2021. Two Putative Cypovirus-Encoded miRNAs Co-regulate the Host Gene of GTP-Binding Nuclear Protein Ran and Facilitate Virus Replication

  23. J Cell Mol Med. 2020 Jul;24(14):8078-8090.doi:10.1111/jcmm.15439. Epub 2020 Jun 10. New mechanism of nerve injury in Alzheimer’s disease: β-amyloid-induced neuronal pyroptosis阿尔茨海默病神经损伤的新机制：神经元下垂

  24. Oncol Rep. 2021 Jun;45(6):95.doi: 10.3892/or.2021.8046. Epub 2021 Apr 13. Progesterone suppresses the progression of colonic carcinoma by increasing the activity of the GADD45α/JNK/c‑Jun signalling pathway黄体酮抑制卵巢癌的进展通过提高结肠癌细胞活性

  25. Front Immunol. 2020 Jun 11;11:1195.doi:10.3389/fimmu.2020.01195. eCollection 2020. Circulating microRNA Expression Profiling Identifies miR-125a-5p Promoting T Helper 1 Cells Response in the Pathogenesis of Hashimoto’s Thyroiditis在桥本氏病的发病机制中

  26. Brain Behav. 2020 Jun;10(6):e01627.doi: 10.1002/brb3.1627. Epub 2020 Apr 19.Mechanism of microRNA-22 in regulating neuroinflammation in Alzheimer’s disease阿尔茨海默病大鼠神经炎症

  27. J Transl Med. 2021 May 26;19(1):223.doi:10.1186/s12967-021-02893-4. miR-20a suppresses Treg differentiation by targeting Map3k9 in experimental autoimmune encephalomyelitis自身免疫性脑脊髓炎

  28. Front Immunol. 2021 May 19;12:668760.doi:10.3389/fimmu.2021.668760. eCollection 2021. Resolvin D1 Improves the Treg/Th17 Imbalance in Systemic Lupus Erythematosus Through miR-30e-5p系统性狼疮失衡

  29. Vet Microbiol. 2020 Mar;242:108610.doi:10.1016/j.vetmic.2020.108610. Epub 2020 Feb 10.Regulatory mechanism of microRNA-155 in chicken embryo fibroblasts in response to reticuloendotheliosis virus infectionmicroRNA-155在鸡胚成纤维细胞中的调控机制

  30. Front Immunol. 2021 Mar 18;12:626310.doi:10.3389/fimmu.2021.626310. eCollection 2021. Adiponectin Enhances B-Cell Proliferation and Differentiation via Activation of Akt1/STAT3 and Exacerbates Collagen-Induced Arthritis加剧胶原诱导的炎症反应

  31. J Extracell Vesicles. 2021Feb;10(4):e12068.doi:10.1002/jev2.12068. Epub 2021 Feb 18.Small extracellular vesicles deliver osteolytic effectors and mediate cancer-induced osteolysis in bone metastatic niche

  32. Am J Transl Res. 2021 Feb 15;13(2):672-683.eCollection 2021. POU2F1 induces the immune escape in lung cancer by up-regulating PD-L1上调PD-L1导致肺癌

  33. Mol Med Rep. 2021 Feb;23(2):154.doi: 10.3892/mmr.2020.11793. Epub 2020 Dec 23. MicroRNA‑223‑induced inhibition of the FBXW7 gene affects the proliferation and apoptosis of colorectal cancer cells via the Notch and Akt/mTOR pathways结直肠癌细胞的增殖和凋亡

  34. Front Med (Lausanne). 2021 Feb 19;8:630962.doi:10.3389/fmed.2021.630962. eCollection 2021. Liraglutide Protects Nucleus Pulposus Cells Against High-Glucose Induced Apoptosis by Activating PI3K/Akt/ mTOR/Caspase-3 and PI3K/Akt/GSK3β/Caspase-3 Signaling Pathways牙髓细胞对高糖的抵抗作用

  35. Theranostics.2021 Jan 1;11(2):893-905.doi: 10.7150/thno.48080. eCollection 2021.uman umbilical cord-derived mesenchymal stem cell therapy ameliorates lupus through increasing CD4+ T cell senescence via MiR-199a-5p/Sirt1/p53 axis人脐带间干细胞治疗通过MiR-199a-5p/Sirt1/p53轴促进CD4+T细胞衰老改善狼疮(IF:11.556).

  36. Life Sci. 2021 Jan 1;264:118687.doi: 10.1016/j.lfs.2020.118687. Epub 2020 Nov 9.PGRN -/- TAMs-derived exosomes inhibit breast cancer cell invasion and migration and its mechanism explorationTAMs衍生的外显体抑制乳腺癌细胞

  37. Toxicol Appl Pharmacol. 2021 Jan 1;410:115336.doi:10.1016/j.taap.2020.115336. Epub 2020 Nov 17.Ligustilide inhibits the proliferation of non-small cell lung cancer via glycolytic metabolism藁本内酯抑制非小细胞肺癌的增殖

  38. Curr Microbiol. 2021 Feb;78(2):490-501. doi: 10.1007/s00284-020-02309-4. Epub 2021 Jan 2. 18 Additional Amino Acids of the Signal Peptide of the Bombyx mori Nucleopolyhedrovirus GP64 Activates Immunoglobulin Binding Protein (BiP) Expression by RNA-seq Ana

  39. J Recept Signal Transduct Res. 2020 Dec;40(6):550-559.doi:10.1080/10799893.2020.1768547. Epub 2020 Jun 1. Antagonizing effect of icaritin on apoptosis and injury of hippocampal neurocytes induced by amyloid beta via GR/BDNF signaling pathway大鼠海马神经细胞损伤的实验研究

  40. Neurochem Res. 2020 Dec;45(12):3045-3058.doi:10.1007/s11064-020-03152-6. Epub 2020 Oct 23. CTRP3 Activates the AMPK/SIRT1-PGC-1α Pathway to Protect Mitochondrial Biogenesis and Functions in Cerebral Ischemic Stroke缺血性脑卒中的线粒体生物发生与功能CTRP3

  41. Sci Rep.2020 Oct 27;10(1):18303.doi: 10.1038/s41598-020-75358-1.MiR-155/GSK-3β mediates anti-inflammatory effect of Chikusetsusaponin IVa by inhibiting NF-κB signaling pathway in LPS-induced RAW264.7 cell.

  42. J Cancer.2020 Feb 3;11(8):2222-2233.doi: 10.7150/jca.39679. eCollection 2020.
    The Role of MiR-5094 as a Proliferation Suppressor during Cellular Radiation Response via Downregulating STAT5b. MiR-5094作为细胞辐射反应中的增殖抑制因子降低STAT5b的表达.

  43. Oncol Lett. 2020 Oct;20(4):68.doi: 10.3892/ol.2020.11929. Epub 2020 Jul 29. Members of the miR-30 family inhibit the epithelial-to-mesenchymal transition of non-small-cell lung cancer cells by suppressing XB130 expression levels抑制非小细胞肺癌细胞的上皮向间充质转化

  44. Mol Ther.2020 Nov 4;28(11):2442-2457.doi: 10.1016/j.ymthe.2020.09.010.Epub 2020 Sep 6.Lactate Induces Production of the tRNA His Half to Promote B-lymphoblastic Cell Proliferation.乳酸诱导tRNA His半体的产生,促进B淋巴细胞增殖.(IF:11.454).

  45. Immunol Lett. 2020 Sep;225:44-49.doi: 10.1016/j.imlet.2020.06.009. Epub 2020 Jun 15. Yes-associated protein upregulates filopodia formation to promote alveolar epithelial-cell phagocytosis肺泡上皮细胞吞噬作用

  46. Mamm Genome.2020 Aug;31(7-8):197-204.doi: 10.1007/s00335-020-09842-4. Epub 2020 Jun 15.ryotic translation initiation factor 3 subunit b is a novel oncogenic factor in prostate cancer

  47. Brain Behav. 2020 Jun; 10(6): e01627.Published online 2020 Apr 19. doi: 10.1002/brb3.1627Mechanism of microRNA‐22 in regulating neuroinflammation in Alzheimer’s disease. microRNA-22调节阿尔茨海默病神经炎症的机制

  48. Cell Stress Chaperones.2020 May;25(3):395-406.doi: 10.1007/s12192-020-01079-z.Epub 2020 Mar 2.Nrf2/ARE is a key pathway for curcumin-mediated protection of TMJ chondrocytes from oxidative stress and inflammation

  49. Glia. 2020 Nov;68(11):2415-2426.doi: 10.1002/glia.23850. Epub 2020 May 27.Activation of astrocytic sigma-1 receptor exerts antidepressant-like effect via facilitating CD38-driven mitochondria transfer星形胶质细胞sigma-1受体的激活

  50. Cell Death Dis.2020 Apr 27;11(4):293.doi: 10.1038/s41419-020-2496-y.CD41-deficient exosomes from non-traumatic femoral head necrosis tissues impair osteogenic differentiation and migration of mesenchymal stem cells.非创伤性股骨头坏死组织中CD41缺陷的外显体影响间充质干细胞的成骨分化和迁移(IF:8.469).

  51. Mol Carcinog.2020 May;59(5):557-568.doi: 10.1002/mc.23180.Epub 2020 Mar 11.XB130, regulated by miR-203, miR-219, and miR-4782-3p, mediates the proliferation and metastasis of non-small-cell lung cancer cells.由miR-203、miR-219和miR-4782-3p调节的XB130介导非小细胞肺癌细胞的增殖和转移

  52. J Nutr.2020 Mar 1;150(3):464-474.doi:10.1093/jn/nxz282.miR-144 Mediates High Fat-Induced Changes of Cholesterol Metabolism via Direct Regulation of C/EBPα in the Liver and Isolated Hepatocytes of Yellow CatfishmiR-144通过直接调节黄鲶肝脏和离体肝细胞中的C/EBPα介导高脂诱导的胆固醇代谢变化

  53. Biochim Biophys Acta Mol Basis Dis. 2019 Dec 1;1865(12):165540. doi: 10.1016/j.bbadis.2019.165540. Epub 2019 Aug 27.(IF:4.328).
    G-MDSC-derived exosomes attenuate collagen-induced arthritis by impairing Th1 and Th17 cell responses.（G-MDSC来源的外体和胶原诱导的关节炎研究）

  54. Cell Cycle.2019 Oct;18(19):2432-2446.doi: 10.1080/15384101.2019.1648956.Epub 2019 Aug 25.lvement of FBP1 in prostate cancer cell epithelial mesenchymal transition, invasion and metastasis by regulating the MAPK signaling pathway

  55. Sci Rep.2019 Nov 22;9(1):17417.doi: 10.1038/s41598-019-53870-3.GF/Flk1 Mechanism is Involved in Roxarsone Promotion of Rat Endothelial Cell Growth and B16F10 Xenograft Tumor Angiogenesis.

  56. Mol Ther Nucleic Acids. 2019 Sep 6;17:38-48. doi: 10.1016/j.omtn.2019.05.004. Epub 2019 May 22.(IF:5.919).
    Circular RNA Expression Profiling and the Potential Role of hsa_circ_0089172 in Hashimoto’s Thyroiditis via Sponging miR125a-3p.（桥本甲状腺炎中cirRNA表达研究）

  57. Biosci Rep. 2019 Aug 19;39(8). pii: BSR20190109. doi: 10.1042/BSR20190109. Print 2019 Aug 30.
    Liraglutide inhibits the apoptosis of human nucleus pulposus cells induced by high glucose through PI3K/Akt/caspase-3 signaling pathway.（利拉鲁肽和人髓核细胞凋亡研究）

  58. J Autoimmun. 2019 Aug;102:96-113. doi: 10.1016/j.jaut.2019.04.018. Epub 2019 May 23.(IF:7.543).
    NF-κB-driven miR-34a impairs Treg/Th17 balance via targeting Foxp3.（mir-34a与Treg/Th17细胞平衡研究）

  59. Cancer Lett. 2019 May 28;450:110-122. doi: 10.1016/j.canlet.2019.02.029. Epub 2019 Feb 18.(IF:6.491).STAT3 inhibition enhances CDN-induced STING signaling and antitumor immunity.(Stat3抑制可增强CDN诱导的STING信号和抗肿瘤免.）

  60. Chemosphere. 2019 May;223:165-170. doi: 10.1016/j.chemosphere.2019.01.169. Epub 2019 Feb 1.(IF:4.427).
    Polychlorinated diphenyl sulfides can induce ROS and genotoxicity via the AhR-CYP1A1 pathway.（多氯代二苯硫醚可通过AHR-CYP1A1途径诱导活性氧和基因毒性.）

  61. Leuk Lymphoma. 2019 Mar;60(3):772-781. doi: 10.1080/10428194.2018.1493727. Epub 2019 Jan 15.
    Targeting the cross-talk between the hedgehog and NF-κB signaling pathways in multiple myeloma.（多发性骨髓瘤中Hedgehog和NF-κB信号通路之间的相互作用.）

  62. Eur Rev Med Pharmacol Sci.2019 Apr;23(8):3291-3301.doi: 10.26355/eurrev_201904_17691.Increased long noncoding RNA LINC00511 is correlated with poor prognosis and contributes to cell proliferation and metastasis by modulating miR-424 in hepatocellular carcinoma.长链非编码RNA LINC00511与肝细胞癌预后不良相关，并通过调节miR-424促进细胞增殖和转移

  63. Mol Cancer. 2019 Mar 30;18(1):61. doi: 10.1186/s12943-019-0978-2.(IF:27.401).
    Long noncoding RNA Pvt1 regulates the immunosuppression activity of granulocytic myeloid-derived suppressor cells in tumor-bearing mice.（LncRNA Pvt1与粒细胞源性骨髓抑制细胞的免疫抑制活性研究）

  64. Int J Mol Med. 2018 Jun;41(6):3379-3393. doi: 10.3892/ijmm.2018.3526. Epub 2018 Mar 1.
    MicroRNA-155 inhibits the osteogenic differentiation of mesenchymal stem cells induced by BMP9 via downregulation of BMP signaling pathway.（间充质干细胞成骨细胞分化研究）

  65. J Virol. 2018 Apr 13;92(9). pii: e00033-18. doi: 10.1128/JVI.00033-18. Print 2018 May 1.
    Lactic acid downregulates viral microRNA to promote Epstein-Barr Virus-immortalized B lymphoblastic cell adhesion and growth.（EB病毒与B淋巴细胞细胞粘附和生长研究）

  66. Mol Med Rep. 2018 Apr;17(4):5851-5859. doi: 10.3892/mmr.2018.8601. Epub 2018 Feb 13.
    Long non‑coding RNA BC168687 small interfering RNA reduces high glucose and high free fatty acid‑induced expression of P2X7 receptors in satellite glial cells.（siRNA对高糖和高游离脂肪酸环境下卫星胶纸细胞P2X7受体表达影响研究）

  67. Biofactors. 2018 Mar;44(2):123-136. doi: 10.1002/biof.1395. Epub 2017 Nov 29.
    Dihydromyricetin inhibits NLRP3 inflammasome-dependent pyroptosis by activating the Nrf2 signaling pathway in vascular endothelial cells.（二氢杨梅素抑制NLRP3炎性体依赖的细胞死亡研究）

  68. Exp Cell Res. 2017 Dec 15;361(2):342-352. doi: 10.1016/j.yexcr.2017.11.002. Epub 2017 Nov 2.
    Erythropoietin ameliorates early brain injury after subarachnoid haemorrhage by modulating microglia polarization via the EPOR/JAK2-STAT3 pathway.（siRNA转染小鼠胶质瘤细胞与促红细胞生成素对早期脑损伤作用研究）

  69. J Cell Mol Med. 2017 Apr;21(4):802-815. doi: 10.1111/jcmm.13022. Epub 2016 Nov 25.
    A novel P53/POMC/Gαs/SASH1 autoregulatory feedback loop activates mutated SASH1 to cause pathologic hyperpigmentation.（SASH1引起病理性色素沉着研究）

  70. J Exp Clin Cancer Res. 2016 Sep 17;35(1):145. doi: 10.1186/s13046-016-0423-y.
    Demystifying the mechanistic and functional aspects of p21 gene activation with double-stranded RNAs in human cancer cells.（p21基因激活与人类癌细胞中dsRNA的机制和功能）

  71. J Orthop Translat. 2016 Jun 8;7:1-6. doi: 10.1016/j.jot.2016.05.011. eCollection 2016 Oct.
    Knockdown of asporin affects transforming growth factor-β1-induced matrix synthesis in human intervertebral annulus cells.（Asporin的敲降可影响人类椎间环细胞中转化生长因子-β1诱导的基质合成）

  72. Immunol Res. 2016 Apr;64(2):489-96. doi: 10.1007/s12026-015-8715-4.
    Upregulation of long noncoding RNA TMEVPG1 enhances T helper type 1 cell response in patients with Sjögren syndrome.（LncRNA-TMEVPG1的上调可增强Sjögren综合征患者T辅助细胞1型细胞反应）

  73. Stem Cells Dev. 2016 Mar 15;25(6):477-91. doi: 10.1089/scd.2015.0301. Epub 2016 Feb 22.
    High-Efficient Transfection of Human Embryonic Stem Cells by Single-Cell Plating and Starvation.（人类胚胎干细胞）

  74. Mol Med Rep. 2016 Mar;13(3):2492-8. doi: 10.3892/mmr.2016.4814. Epub 2016 Jan 27.
    Downregulated microRNA-23b promotes BMP9-mediated osteogenesis in C2C12 myoblast cells by targeting Runx2.（下调的microRNA-23b通过靶向Runx2促进C2C12成肌细胞中BMP9介导的骨生成）

  75. Int J Mol Med. 2015 Dec;36(6):1497-506. doi: 10.3892/ijmm.2015.2363. Epub 2015 Oct 9.
    miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells.（miR-21激活小鼠干细胞中BMP9 /Smad信号通路与BMP9 协同作用于成骨细胞分化的研究）

  76. Mol Biosyst. 2015 Nov;11(11):3040-7. doi: 10.1039/c5mb00347d.
    The involvement of aquaporin 1 in the hepatopulmonary syndrome rat serum-induced migration of pulmonary arterial smooth muscle cells via the p38-MAPK pathway.（肝肺综合征相关的研究）

  77. Tumour Biol. 2016 Mar;37(3):3479-88. doi: 10.1007/s13277-015-4086-7. Epub 2015 Oct 9.
    MiR-30a regulates the proliferation, migration, and invasion of human osteosarcoma by targeting Runx2.（MiR-30a通过靶向作用于小家畜相关的转录因子2（Runx2）进而调节人骨肉瘤细胞的增值、迁移及侵袭）

  78. Endocrine. 2015 Aug;49(3):752-60. doi: 10.1007/s12020-015-0546-5. Epub 2015 Feb 11.
    MiR-346 regulates CD4⁺CXCR5⁺ T cells in the pathogenesis of Graves’ disease.（甲亢研究）

  79. J Immunol. 2015 Aug 1;195(3):1301-11. doi: 10.4049/jimmunol.1500209. Epub 2015 Jun 19.
    MicroRNA-9 Regulates the Differentiation and Function of Myeloid-Derived Suppressor Cells via Targeting Runx1.（MicroRNA-9 通过靶向作用于Runx1调节髓源性抑制细胞的分化和功能）

  80. Immunol Res. 2015 Jun;62(2):129-36. doi: 10.1007/s12026-015-8643-3.
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应用本产品文献

  1. EntransterTM系列转染试剂的成分和原理，化学结构，粒径，电位等数据

  2. siRNA转染细胞铺板密度为什么重要？

  3. siRNA转染后什么时候做qRT-PCR最好？ siRNA转染后什么时候做蛋白检测检测最好？

  4. siRNA转染与DNA转染有什么不一样?

  5. 成功siRNA转染的主要关键点有什么？

  6. 为什么EntransterTM转染试剂有血清转染不仅没有毒性，反而有助于提高转染效率?

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