订单满1000元，免运费
EntransterTM-H4000是英格恩生物公司针对常规转染和工业转染中转染量大，要求高，花费大，而且不宜随便更改方法的特点研制的。 该试剂与常见的脂质体试剂用法和用量一样，但效率更高，同时，每支更节省1000元以上。 非常适合对转染质量和价格有较高要求的常规转染实验和工业转染。
常规转染实验。 工业转染。
| 人源，遗传变异细胞和正常组织来源细胞 | 人源，肿瘤细胞 | 其他动物来源细胞 |
| AsPC-1，BNL CL2，HEK293，CFPEo，HUVEC，HLMEC，H-MVEC，MRC-5，HCS-2/8，HLF，D407，H9C2，SMC | A549，HuH-7，HepG2，HME4，A375，Hep-2，SW480，SKOV-3，IGROV1，K562，Jurkat，MCF7，MDA-MB-231，ZR-75-1，TCA8113，Eca-109，SGC-7901，HeLa，SiHa，Ca Ski，CaCo2，SAOS-2，PC-3，SHEP | B16BL6，B16F10，C2C12，C6，CHO，COS-7，CV-1，BHK-21，MDCK，PC12，BAEC，MLE-12，MLE-15，Neuro2A，PGHAM-1，RAW 264.7，EL-4，L929，LLC，3LL，NIH 3T3 |
各细胞系详细转染效率情况
| 细胞名称 | 来源 | 转染效率(%) |
| AsPC-1 | 人源，胰脏细胞 | 80% |
| BNL CL2 | 人源，胚胎肾细胞 | 70% |
| HEK 293 | 人源，胚胎肾细胞 | 90% |
| CFPEo | 人源，气管上皮细胞 | 50% |
| HUVEC | 人源，脐带静脉内皮状细胞 | 55% |
| HLMEC | 人源，肺血管内皮细胞 | 60% |
| H-MVEC | 人源，微血管内皮细胞 | 50% |
| MRC-5 | 人源，胚胎肺细胞 | 75% |
| HCS-2/8 | 人源，软骨样细胞 | 40% |
| HLF | 人源，肺成纤维细胞 | 65% |
| D 407 | 人源，视网膜色素上皮细胞 | 60% |
| A549 | 人源，肺癌细胞 | 90% |
| HuH-7 | 人源，肝癌细胞 | 63% |
| HepG2 | 人源，肝癌细胞 | 30% |
| HME4 | 人源，黑色素瘤细胞 | 90% |
| A375 | 人源，黑色素瘤细胞 | 70% |
| Hep-2 | 人源，喉癌细胞 | 70% |
| SAOS-2 | 人源，骨肉瘤细胞 | 50% |
| SKOV-3 | 人源，卵巢腺癌细胞 | 60% |
| IGROV1 | 人源，卵巢癌细胞 | 20% |
| K562 | 人源，慢性白血病细胞 | 30% |
| Jurkat | 人源，T淋巴细胞白血病细胞 | 45% |
| MCF7 | 人源，乳腺癌细胞 | 55% |
| MDA-MB-231 | 人源，乳腺癌细胞 | 70% |
| ZR-75-1 | 人源，乳腺癌细胞 | 70% |
| TCA8113 | 人源，舌鳞癌细胞 | 90% |
| Eca-109 | 人源，胃腺癌细胞 | 57% |
| SGC-7901 | 人源，胃腺癌细胞 | 48% |
| BGC-823 | 人源，胃腺癌细胞 | 60% |
| Panc-1 | 人源，胰腺癌细胞 | 40% |
| HeLa | 人源，宫颈癌细胞 | 80% |
| SiHa | 人源，宫颈癌细胞 | 70% |
| Ca Ski | 人源，宫颈表皮样癌细胞 | 80% |
| CaCo2 | 人源，结肠癌上皮细胞 | 30% |
| SW480 | 人源，结肠腺癌细胞 | 80% |
| PC-3 | 人源，前列腺癌细胞 | 30% |
| SHEP | 人源，神经母细胞瘤细胞 | 70% |
| B16BL6 | 小鼠，黑色素瘤细胞 | 90% |
| B16F10 | 小鼠，黑色素瘤细胞 | 80% |
| C2C12 | 小鼠，胚胎肌原细胞 | 30% |
| C6 | 大鼠，脑胶质瘤细胞 | 60% |
| CHO | 中国仓鼠，卵巢细胞 | 90% |
| COS-7 | 猴SV40转化肾细胞 | 80% |
| CV-1 | 非洲绿猴肾细胞 | 70% |
| BHK-21 | 仓鼠，肾细胞 | 80% |
| MDCK | 犬，肾上皮细胞 | 30% |
| PC12 | 大鼠，肾上腺嗜铬细胞瘤细胞 | 33% |
| BAEC | 牛，动脉内皮细胞 | 50% |
| MLE-12 | 小鼠，肺上皮细胞 | 70% |
| MLE-15 | 小鼠，肺上皮细胞 | 70% |
| Neuro2A | 小鼠，成神经瘤细胞 | 70% |
| PGHAM-1 | 仓鼠，胰腺癌细胞 | 90% |
| RAW 264.7 | 小鼠，巨噬细胞 | 15% |
| EL-4 | 小鼠，胸腺瘤细胞 | 75% |
| L929 | 小鼠，成纤维肉瘤细胞 | 60% |
| LLC | 小鼠，路易斯肺癌细胞 | 75% |
| 3LL | 小鼠，路易斯肺癌细胞 | 65% |
| NIH 3T3 | 小鼠，胚胎纤维原细胞 | 40% |
| P19 | 小鼠，胚胎肉瘤细胞 | 60% |
用法用量和常见的脂质体试剂完全一样，可不改流程方法更换试剂 转染效率更高，每支节省1000元以上，可根据需要定做500ml以上大包装，可批量定制产品。
Biomed Pharmacother. 2021 Oct;142:112056.doi:10.1016/j.biopha.2021.112056. Epub 2021 Aug 23. Dysregulation of miR-342-3p in plasma exosomes derived from convalescent AMI patients and its consequences on cardiac repair恢复期AMI患者血浆外小体中miR-342-3p的失调及其对心脏修复的影响
Cell Biosci. 2021 Aug 4;11(1):156.doi: 10.1186/s13578-021-00669-3. Modaline sulfate promotes Oct4 expression and maintains self-renewal and pluripotency of stem cells through JAK/STAT3 and Wnt signaling pathways通过JAKSTAT3和Wnt检测干细胞
Front Physiol. 2021 Aug 4;12:663482.doi:10.3389/fphys.2021.663482. eCollection 2021. Two Putative Cypovirus-Encoded miRNAs Co-regulate the Host Gene of GTP-Binding Nuclear Protein Ran and Facilitate Virus ReplicationmiRNA共同调节宿主基因GTP结合核蛋白Ran的表达
Cell Death Differ. 2021 Jul;28(7):2160-2178.doi:10.1038/s41418-021-00744-9. Epub 2021 Mar 4. MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasmMACF1通过隔离促进成骨细胞分化
Genet Test Mol Biomarkers. 2021 Jun;25(6):399-404.doi: 10.1089/gtmb.2021.0019. Epub 2021 Jun 2.Whole-Exome Sequencing Reveals Novel TSPAN12 Variants in Autosomal Dominant Familial Exudative Vitreoretinopathy
Front Pharmacol. 2021 Jun 14;12:696729.doi:10.3389/fphar.2021.696729. eCollection 2021. Forsythiasides-Rich Extract From Forsythiae Fructus Inhibits Mast Cell Degranulation by Enhancing Mitochondrial Ca 2+ Uptake连翘抑制肥大细胞生长线粒体钙摄取
Biochem Pharmacol. 2021 Apr;186:114476.doi:10.1016/j.bcp.2021.114476. Epub 2021 Feb 17. Aloe-emodin, a naturally occurring anthraquinone, is a highly potent mast cell stabilizer through activating mitochondrial calcium uniporter芦荟大黄素
Gene. 2021 Apr 20;777:145450.doi: 10.1016/j.gene.2021.145450. Epub 2021 Jan 29. A 14-amino acids deletion in BmShadow results to non-moult on the 2nd instar in the bivoltine silkworm, Bombyx moriBmShadow中14个氨基酸的缺失导致二伏家蚕2龄时不蜕皮
Pharmacol Res. 2021 Jan;163:105230.doi:10.1016/j.phrs.2020.105230. Epub 2020 Oct 5. Long noncoding RNA AK039312 and AK079370 inhibits bone formation via miR-199b-5p长非编码RNA AK039312和AK079370抑制骨形成
Pharmacol Res. 2020 Oct 5;105230. doi: 10.1016/j.phrs.2020.105230 Long noncoding RNA AK039312 and AK079370 inhibits bone formation via miR-199b-5p
Biochem Biophys Res Commun. 2020 Dec10;533(3):613-619.doi:10.1016/j.bbrc.2020.09.063. Epub 2020 Sep 25. Prefoldin subunit MM1 promotes cell migration via facilitating filopodia formation预折叠蛋白亚单位MM1通过促进细胞迁移
Graefes Arch Clin Exp Ophthalmol. 2020 Oct;258(10):2251-2261.doi: 10.1007/s00417-020-04636-5.Epub 2020 Jun 7.Disease-causing mutations associated with bestrophinopathies promote apoptosis in retinal pigment epithelium cells.视网膜色素上皮细胞凋亡
Biochem Pharmacol. 2020 Jul;177:113984.doi:10.1016/j.bcp.2020.113984. Epub 2020 Apr 18. Ethyl-p-methoxycinnamate enhances oct4 expression and reinforces pluripotency through the NF-κB signaling pathway对甲氧基肉桂酸乙酯增强oct4表达并增强多能性
Small.2020 Jul;16(30):e1907301.doi: 10.1002/smll.201907301. Epub 2020 Jun 25.Photoactivatable RNA N 6 -Methyladenosine Editing with CRISPR-Cas13甲基腺苷编辑(IF:13.281).
Front Genet.2020 May 26;11:511.doi: 10.3389/fgene.2020.00511. eCollection 2020.MICA ∗ 012:01 Allele Facilitates the Metastasis of KRAS-Mutant Colorectal Cancer.KRAS突变型结直肠癌的转移
J Cell Biochem. 2019 Sep;120(9):14326-14335.doi: 10.1002/jcb.28538. Epub 2019 May 20. Bom-miR-2805 upregulates the expression of Bombyx mori fibroin light chain gene in vivo. Bom-miR-2805上调体内家蚕丝素轻链基因的表达。
Genes Dis.2019 Sep 17;8(1):94-109.doi: 10.1016/j.gendis.2019.09.005. eCollection 2021 Jan.SFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS.SRSFs介导多囊卵巢综合征患者卵巢颗粒细胞AR的功能
J Cell Physiol . 2019 Jul;234(7):11524-11536. doi: 10.1002/jcp.27815. Epub 2019 Jan 17. A novel long noncoding RNA AK016739 inhibits osteoblast differentiation and bone formation
Eur Rev Med Pharmacol Sci.2019 Apr;23(8):3291-3301.doi: 10.26355/eurrev_201904_17691.Increased long noncoding RNA LINC00511 is correlated with poor prognosis and contributes to cell proliferation and metastasis by modulating miR-424 in hepatocellular carcinoma.长链非编码RNA LINC00511与肝细胞癌预后不良相关，并通过调节miR-424促进细胞增殖和转移
ACS Sens.2019 Apr 26;4(4):977-983.doi: 10.1021/acssensors.9b00102. Epub 2019 Mar 21.Rapidly Visualizing the Membrane Affinity of Gene Vectors Using Polydiacetylene-Based Allochroic Vesicles.(IF:7.711).
Gene.2019 Mar10;688:98-106. doi:10.1016/j.gene.2018.11.095.
Embryonic morphology observation and HOXC8 gene expression in crest cushions of Chinese Crested duck
J Cell Physiol . 2018 Jul;233(7):5405-5419. doi: 10.1002/jcp.26374. Epub 2018 Jan 23. Mechanical unloading reduces microtubule actin crosslinking factor 1 expression to inhibit β-catenin signaling and osteoblast proliferation
Oncol Lett . 2018 Apr;15(4):4344-4350. doi: 10.3892/ol.2018.7831. Epub 2018 Jan 19. Synergistic effect of receptor-interacting protein 140 and simvastatin on the inhibition of proliferation and survival of hepatocellular carcinoma cells
Toxicol Appl Pharmacol . 2017 May 1;322:75-88. doi: 10.1016/j.taap.2017.03.003. Epub 2017 Mar 8. Molecular mechanisms of 3,3’4,4′,5-pentachlorobiphenyl-induced epithelial-mesenchymal transition in human hepatocellular carcinoma cells
Mol Med Rep . 2016 Feb;13(2):1725-31. doi: 10.3892/mmr.2015.4704. Epub 2015 Dec 22. Myricetin inhibits Kv1.5 channels in HEK293 cells.
Nat Commun . 2015 Aug 24;6:8022. Rhomboid domain containing 1 promotes colorectal cancer growth through activation of the EGFR signalling pathway. (IF:11.329).
Mol Cell Biol . 2015 Jul;35(14):2400-13. doi: 10.1128/MCB.00090-15. Epub 2015 May 4. SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity
Oncotarget . 2015 May 30;6(15):13049-59. Wedelolactone disrupts the interaction of EZH2-EED complex and inhibits PRC2-dependent cancer. (IF:6.359).
Eur J Med Chem . 2014 Oct 6;85:119-26. doi: 10.1016/j.ejmech.2014.07.091. Epub 2014 Jul 25. Discovery of highly potent TNFα inhibitors using virtual screen.
Biochem Biophys Res Commun . 2014 Apr 4;446(2):523-8. doi: 10.1016/j.bbrc.2014.02.137. Epub 2014 Mar 12. eEF1Bγ is a positive regulator of NF-кB signaling pathway.
Int J Nanomedicine . 2013;8:3805-16. doi: 10.2147/IJN.S48950. Epub 2013 Oct 2. Efficacy and safety of dendrimer nanoparticles with coexpression of tumor necrosis factor-α and herpes simplex virus thymidine kinase in gene radiotherapy of the human uveal melanoma OCM-1 cell line. (IF:6.400).
J Immunol . 2013 Sep 1;191(5):2614-23. doi: 10.4049/jimmunol.1203143. Epub 2013 Aug 5. Enhancer of zeste homolog 2 is a negative regulator of mitochondria-mediated innate immune responses.
Appl Microbiol Biotechnol . 2013 Jul;97(14):6301-14. doi: 10.1007/s00253-013-4787-z. Epub 2013 Mar 6. Design, expression and characterization of a novel coexpression system of two antiarthritic molecules.
Heart Vessels . 2013 May;28(3):385-96. doi: 10.1007/s00380-012-0275-0. Epub 2012 Sep 1. Real-time tracking of adipose tissue-derived stem cells with injectable scaffolds in the infarcted heart.
PLoS One . 2012;7(11):e49567. doi:10.1371/journal.pone.0049567. Epub 2012 Nov 19. The ubiquitin ligase TRAF6 negatively regulates the JAK-STAT signaling pathway by binding to STAT3 and mediating its ubiquitination.
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