长核酸转染试剂EntransterTM-H4000

货号规格单价数量
4000-50.75ml¥999.00

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4000-61.5ml¥1,799.00

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订单满1000元,免运费

产品介绍

EntransterTM-H4000是英格恩生物公司研发合成的纳米聚合物转染试剂,该试剂采用纳米技术合成,是最新一代非病毒转染试剂。由于纳米技术的应用,EntransterTM-H4000在细胞转染过程中,表现了卓越的低毒、高效的性能。

本品推荐用于常规DNA以及长片段(>1kb)的mRNA等RNA的转染。

推荐应用范围

常规DNA以及长片段(>1kb)的mRNA等RNA的转染。

成功转染的细胞

人源,遗传变异细胞和正常组织来源细胞 人源,肿瘤细胞 其他动物来源细胞
AsPC-1,BNL CL2,HEK293,CFPEo,HUVEC,HLMEC,H-MVEC,MRC-5,HCS-2/8,HLF,D407,H9C2,SMC A549,HuH-7,HepG2,HME4,A375,Hep-2,SW480,SKOV-3,IGROV1,K562,Jurkat,MCF7,MDA-MB-231,ZR-75-1,TCA8113,Eca-109,SGC-7901,HeLa,SiHa,Ca Ski,CaCo2,SAOS-2,PC-3,SHEP B16BL6,B16F10,C2C12,C6,CHO,COS-7,CV-1,BHK-21,MDCK,PC12,BAEC,MLE-12,MLE-15,Neuro2A,PGHAM-1,RAW 264.7,EL-4,L929,LLC,3LL,NIH 3T3

各细胞系详细转染效率情况

细胞名称 来源 转染效率(%)
AsPC-1 人源,胰脏细胞 80%
BNL CL2 人源,胚胎肾细胞 70%
HEK 293 人源,胚胎肾细胞 90%
CFPEo 人源,气管上皮细胞 50%
HUVEC 人源,脐带静脉内皮状细胞 55%
HLMEC 人源,肺血管内皮细胞 60%
H-MVEC 人源,微血管内皮细胞 50%
MRC-5 人源,胚胎肺细胞 75%
HCS-2/8 人源,软骨样细胞 40%
HLF 人源,肺成纤维细胞 65%
D 407 人源,视网膜色素上皮细胞 60%
A549 人源,肺癌细胞 90%
HuH-7 人源,肝癌细胞 63%
HepG2 人源,肝癌细胞 30%
HME4 人源,黑色素瘤细胞 90%
A375 人源,黑色素瘤细胞 70%
Hep-2 人源,喉癌细胞 70%
SAOS-2 人源,骨肉瘤细胞 50%
SKOV-3 人源,卵巢腺癌细胞 60%
IGROV1 人源,卵巢癌细胞 20%
K562 人源,慢性白血病细胞 30%
Jurkat 人源,T淋巴细胞白血病细胞 45%
MCF7 人源,乳腺癌细胞 55%
MDA-MB-231 人源,乳腺癌细胞 70%
ZR-75-1 人源,乳腺癌细胞 70%
TCA8113 人源,舌鳞癌细胞 90%
Eca-109 人源,胃腺癌细胞 57%
SGC-7901 人源,胃腺癌细胞 48%
BGC-823 人源,胃腺癌细胞 60%
Panc-1 人源,胰腺癌细胞 40%
HeLa 人源,宫颈癌细胞 80%
SiHa 人源,宫颈癌细胞 70%
Ca Ski 人源,宫颈表皮样癌细胞 80%
CaCo2 人源,结肠癌上皮细胞 30%
SW480 人源,结肠腺癌细胞 80%
PC-3 人源,前列腺癌细胞 30%
SHEP 人源,神经母细胞瘤细胞 70%
B16BL6 小鼠,黑色素瘤细胞 90%
B16F10 小鼠,黑色素瘤细胞 80%
C2C12 小鼠,胚胎肌原细胞 30%
C6 大鼠,脑胶质瘤细胞 60%
CHO 中国仓鼠,卵巢细胞 90%
COS-7 猴SV40转化肾细胞 80%
CV-1 非洲绿猴肾细胞 70%
BHK-21 仓鼠,肾细胞 80%
MDCK 犬,肾上皮细胞 30%
PC12 大鼠,肾上腺嗜铬细胞瘤细胞 33%
BAEC 牛,动脉内皮细胞 50%
MLE-12 小鼠,肺上皮细胞 70%
MLE-15 小鼠,肺上皮细胞 70%
Neuro2A 小鼠,成神经瘤细胞 70%
PGHAM-1 仓鼠,胰腺癌细胞 90%
RAW 264.7 小鼠,巨噬细胞 15%
EL-4 小鼠,胸腺瘤细胞 75%
L929 小鼠,成纤维肉瘤细胞 60%
LLC 小鼠,路易斯肺癌细胞 75%
3LL 小鼠,路易斯肺癌细胞 65%
NIH 3T3 小鼠,胚胎纤维原细胞 40%
P19 小鼠,胚胎肉瘤细胞 60%

主要特点

用法用量和常见的脂质体试剂完全一样,可不改流程方法更换试剂 转染效率更高,每支节省1000元以上,可根据需要定做500ml以上大包装,可批量定制产品。

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  1. EntransterTM系列转染试剂的成分和原理,化学结构,粒径,电位等数据

  2. 为什么EntransterTM转染试剂有血清转染不仅没有毒性,反而有助于提高转染效率?

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运输与储存

常温运输,于4℃长期存储。

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本品使用安全,未发现任何生物、化学毒性。如不慎沾染,用清水冲洗即可。

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