RNA转染试剂EntransterTM-R4000

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4000-30.75ml¥1,199.00

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4000-41.5ml¥1,999.00

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产品介绍

EntransterTM-R4000是英格恩生物(Engreen Biosystem)最新研发合成的针对siRNA、microRNA、mimic、inhibitor、mRNA和shRNA等RNA的转染试剂。EntransterTM-R4000不仅可以转染小RNA,而且针对mRNA等长链RNA优化。由于纳米技术的应用,EntransterTM-R4000能做到高达90%的转染效率和60-90%的沉默效率,对难转染细胞和原代细胞也非常适用。

推荐应用范围

小RNA转染。包括siRNA转染,miRNA,mimic,inhibitor等。 长链的mRNA和shRNA转染,针对mRNA和长链的shRNA等RNA转染优化。 可转染动物细胞、昆虫细胞等,也可以转染原代细胞、悬浮细胞和原代细胞等传统难转染细胞。

主要特点

纳米聚合物,转染效果优于脂质体转染试剂 高效转染,沉默效率高,可以达到90%以上的沉默效率 低细胞毒性,有血清转染,无须反复更换培养基 原代细胞、悬浮细胞和敏感细胞都能转。

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    Erythropoietin ameliorates early brain injury after subarachnoid haemorrhage by modulating microglia polarization via the EPOR/JAK2-STAT3 pathway.(siRNA转染小鼠胶质瘤细胞与促红细胞生成素对早期脑损伤作用研究)

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    A novel P53/POMC/Gαs/SASH1 autoregulatory feedback loop activates mutated SASH1 to cause pathologic hyperpigmentation.(SASH1引起病理性色素沉着研究)

  15. J Exp Clin Cancer Res. 2016 Sep 17;35(1):145. doi: 10.1186/s13046-016-0423-y.
    Demystifying the mechanistic and functional aspects of p21 gene activation with double-stranded RNAs in human cancer cells.(p21基因激活与人类癌细胞中dsRNA的机制和功能)

  16. J Orthop Translat. 2016 Jun 8;7:1-6. doi: 10.1016/j.jot.2016.05.011. eCollection 2016 Oct.
    Knockdown of asporin affects transforming growth factor-β1-induced matrix synthesis in human intervertebral annulus cells.(Asporin的敲降可影响人类椎间环细胞中转化生长因子-β1诱导的基质合成)

  17. Immunol Res. 2016 Apr;64(2):489-96. doi: 10.1007/s12026-015-8715-4.
    Upregulation of long noncoding RNA TMEVPG1 enhances T helper type 1 cell response in patients with Sjögren syndrome.(LncRNA-TMEVPG1的上调可增强Sjögren综合征患者T辅助细胞1型细胞反应)

  18. Stem Cells Dev. 2016 Mar 15;25(6):477-91. doi: 10.1089/scd.2015.0301. Epub 2016 Feb 22.
    High-Efficient Transfection of Human Embryonic Stem Cells by Single-Cell Plating and Starvation.(人类胚胎干细胞)

  19. Mol Med Rep. 2016 Mar;13(3):2492-8. doi: 10.3892/mmr.2016.4814. Epub 2016 Jan 27.
    Downregulated microRNA-23b promotes BMP9-mediated osteogenesis in C2C12 myoblast cells by targeting Runx2.(下调的microRNA-23b通过靶向Runx2促进C2C12成肌细胞中BMP9介导的骨生成)

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    miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells.(miR-21激活小鼠干细胞中BMP9 /Smad信号通路与BMP9 协同作用于成骨细胞分化的研究)

  21. Mol Biosyst. 2015 Nov;11(11):3040-7. doi: 10.1039/c5mb00347d.
    The involvement of aquaporin 1 in the hepatopulmonary syndrome rat serum-induced migration of pulmonary arterial smooth muscle cells via the p38-MAPK pathway.(肝肺综合征相关的研究)

  22. Tumour Biol. 2016 Mar;37(3):3479-88. doi: 10.1007/s13277-015-4086-7. Epub 2015 Oct 9.
    MiR-30a regulates the proliferation, migration, and invasion of human osteosarcoma by targeting Runx2.(MiR-30a通过靶向作用于小家畜相关的转录因子2(Runx2)进而调节人骨肉瘤细胞的增值、迁移及侵袭)

  23. Endocrine. 2015 Aug;49(3):752-60. doi: 10.1007/s12020-015-0546-5. Epub 2015 Feb 11.
    MiR-346 regulates CD4⁺CXCR5⁺ T cells in the pathogenesis of Graves’ disease.(甲亢研究)

  24. J Immunol. 2015 Aug 1;195(3):1301-11. doi: 10.4049/jimmunol.1500209. Epub 2015 Jun 19.
    MicroRNA-9 Regulates the Differentiation and Function of Myeloid-Derived Suppressor Cells via Targeting Runx1.(MicroRNA-9 通过靶向作用于Runx1调节髓源性抑制细胞的分化和功能)

  25. Immunol Res. 2015 Jun;62(2):129-36. doi: 10.1007/s12026-015-8643-3.
    Decreased expression of microRNA-125a-3p upregulates interleukin-23 receptor in patients with Hashimoto’s thyroiditis.(microRNA-125a-3p的低表达可以上调淋巴瘤性(样)甲状腺肿病人白细胞介素-23受体的水平).

  26. PLoS One. 2015 Mar 31;10(3):e0121510. doi: 10.1371/journal.pone.0121510. eCollection 2015.
    MicroRNA-22 impairs anti-tumor ability of dendritic cells by targeting p38.(MicroRNA-22 通过靶向作用于p38蛋白削弱树突状细胞的抗肿瘤能力)

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    RIP1-dependent Bid cleavage mediates TNFα-induced but Caspase-3-independent cell death in L929 fibroblastoma cells.(成纤维细胞瘤细胞研究)

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    Paxillin suppresses the proliferation of HPS rat serum treated PASMCs by up-regulating the expression of cytoskeletal proteins.

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    MicroRNA-148b enhances the radiosensitivity of non-Hodgkin’s Lymphoma cells by promoting radiation-induced apoptosis.

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应用本产品文献

  1. EntransterTM系列转染试剂的成分和原理?

  2. siRNA转染细胞铺板密度为什么重要?

  3. siRNA转染后什么时候做qRT-PCR最好? siRNA转染后什么时候做蛋白检测检测最好?

  4. siRNA转染与DNA转染有什么不一样?

  5. 成功siRNA转染的主要关键点有什么?

  6. 为什么EntransterTM转染试剂有血清转染不仅没有毒性,反而有助于提高转染效率?

相关问题

运输与储存

常温运输,储存于4℃。

安全性

本品使用安全,未发现任何生物、化学毒性。如不慎沾染,用清水冲洗即可。

质量保证

北京英格恩生物科技有限公司本产品实行严格质量检验,并进行转染验证,以确保产品质量。请用户使用前务必认真阅读操作手册。如用户仍然有问题,请立即联系本公司人员,英格恩公司将负责提供全程技术服务。

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